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Enzyme linked Immunosorbent Assays were first developed in the 1960s and 70s and have continued to be honed to greater sensitivity and applicability. ELISA assays are used to measure the amount of allergen present in a particular sample. At AHG our biochemists run routine ELISAs for various allergenic molecules such as house dust mite, animal and various pollen allergens. Where necessary, augmented assays can be performed to measure down to even more sensitive levels.
As well as routine ELISA testing, AHG’s research biochemists have the skills and experience to develop new tests for a variety of purposes. They can also work with clients to extract value and information from relevant testing protocols.
The basic premise of an ELISA is as follows. An antibody to the antigen of interest in bound to a plastic well. The sample (possibly containing the antigen of interest) is added to the well and allowed time to bind. A wash step is included and any unbound allergen is removed. At this stage the same antibody is added but this time it has an enzyme linked to it. If the antigen is present and bound to the first antibody, the second enzyme linked antibody will bind. Unbound second antibody is washed away. A reagent is added that catalyses a colour reaction when the enzyme is present, thus ultimately proving that the allergen is present, the amount of colour produced is proportional to the amount of antigen present.
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