JIS Z 2801 – Testing for Antimicrobial Activity and Efficacy

Overview

The JIS Z 2801 standard outlines a method for testing the antimicrobial activity and efficacy of surfaces treated with antimicrobial agents, focusing on the reduction of bacterial populations. This standard is widely used for testing the antibacterial properties of non-porous surfaces like plastics.

• Products That Can Be Tested

  • Plastics: Various plastic surfaces that require antimicrobial properties can be evaluated using this method.

Test Description

The JIS Z 2801 standard evaluates the antimicrobial properties of treated surfaces by determining the reduction in viable bacteria over a set period of time. The test involves comparing a treated surface against an untreated control surface, both of which are inoculated with a specific bacterial culture.

The key steps in the test method are as follows:

1. Test Organisms
   Two bacterial strains are used for testing:
   • Escherichia coli (ATCC 25922/ATCC 8739)
   • Staphylococcus aureus (ATCC 6538/ATCC 6538p)

   The bacteria are grown at 37°C for 24 hours and diluted to a working concentration of 5 x 10^5 cells/ml.

2. Inoculation
   The control and test surfaces are inoculated with the bacterial culture. A sterile film is placed over the inoculated surfaces to ensure even and consistent contact between the surface and the bacterial suspension.
3. Incubation
   The samples are incubated at 37°C for 18 hours.
4. Bacterial Viability Determination
   At the start of the test (t = 0), bacterial viability is determined by serial dilution and plating onto agar to enumerate colony-forming units (CFU). After incubation, the bacterial populations are recovered by washing the test surfaces, and the extraction solution is serially diluted and plated on agar.
5. Neutralization Validation
   A control is used to validate the elution method, ensuring that the antimicrobial agent is effectively neutralized, which prevents it from affecting bacterial recovery.
6. Efficacy Calculation
   The antimicrobial efficacy is determined by calculating the log reduction in bacterial viability on the treated sample compared to the untreated control. A higher log reduction indicates greater antimicrobial efficacy.

Key Benefits of JIS Z 2801 Testing

• Provides a reliable method for evaluating the antibacterial properties of non-porous surfaces.
• Ensures scientifically validated data on bacterial reduction, supporting product claims.
• Allows manufacturers to test product performance against both E. coli and S. aureus, which are commonly associated with healthcare and consumer products.

Why Choose Airmid Health Labs for JIS Z 2801  Testing?

At Airmid Health Labs, we provide reliable, third-party validation for the virucidal efficacy of disinfectants and antiseptics. Our testing services help manufacturers meet industry standards and build consumer trust through scientifically backed claims.

Upon successful completion of the JIS Z 2801 test, the product can be labeled as tested by Airmid Health Labs to JIS Z 2801 standards. This third-party validation provides scientific support for any claims related to the antimicrobial efficacy of the product.

Turnaround Time

At Airmid Health Labs, we understand the importance of delivering timely results. Our testing typically has a turnaround time of 4-6 weeks, ensuring that you can move forward with product development and marketing efficiently.

Get in Touch

Contact Airmid Health Labs today to discuss your testing needs and to request a quote for JIS Z 2801 testing