AATCC 100 Fabric Testing and Claim Verification
Scope of the AATCC 100 Test Method
The AATCC 100 test method evaluates the antibacterial activity of textile materials treated with antimicrobial finishes. It is one of the most widely recognized standards for determining the effectiveness of antibacterial treatments applied to fabrics and other textile products.

Products That Can Be Tested
Woven and nonwoven textiles
Garments, upholstery, and bedding fabrics
Treated fibres and coatings designed to inhibit bacterial growth
Description of the AATCC 100 Test
The AATCC 100 antibacterial test measures the ability of treated textiles to reduce bacterial populations under controlled laboratory conditions.
Two representative microorganisms—Staphylococcus aureus and Klebsiella pneumoniae—are used as test organisms. The adjusted bacterial suspension is applied to both treated and untreated textile specimens.
At time zero (t = 0) and after a specified contact period, 10 ml of neutralising solution are added to the test materials to extract the bacteria. The extracted solutions are serially diluted and plated on nutrient agar to determine the number of colony forming units (CFUs) present.
The antibacterial efficacy of the finish is expressed as the percentage reduction or log reduction in bacterial count compared with the untreated control fabric. This quantifies the performance of the antimicrobial treatment in reducing viable bacteria on the textile surface.
Independent Validation
On successful completion, products can be described as tested by Airmid Healthgroup to AATCC 100, providing independent validation in support of claims relating to antibacterial performance and data obtained.
Airmid Healthgroup’s ISO/IEC 17025–accredited laboratory provides third-party testing services trusted by manufacturers, regulators, and certification programs worldwide.
Turnaround Time of the AATCC 100 Test
Standard turnaround time is approximately 4 weeks from receipt of samples.
Related Tests and Sevices at Airmid Healthgroup
ISO 20743:2021 standard outlines a test method to determine the antibacterial activity of textile products
ISO 9237 standard outlines a test method for determining the air permeability of textiles
Learn more about our GLP-compliant environmental chamber testing
Related Blogs and Case Studies
Airmid Healthgroup validates Chem-Dry’s cleaning process, showing 89% airborne bacteria reduction for healthier indoor spaces.
Beko’s HygieneShield™ range, validated by Airmid Healthgroup, eliminates bacteria and viruses using UV light and high-temperature technology for enhanced home hygiene
- Airmid Validates Light-Activated Antibacterial Tiles by VitrA as 99.9% Effective Against MRSA, E. Coli, and Athlete’s Foot Fungus
Airmid Healthgroup validated VitrA’s light-activated antibacterial tiles as 99.9% effective against MRSA, E. Coli, and Athlete’s Foot fungus, supporting their market entry for healthcare and swimming pools.
Textile Testing Menu
- Allergen Barrier Test
- Simulated Use Test
- Allergen Removal
- Heat Escape Method
- AATCC 194-2013
- Breathability
- Air Permeability
- Water Resistance
- ISO 27447:2019
- ISO 20743:2021
- BS EN 14683:2019
- AATCC 100
- Microbial Strikethrough
- Feather & Down Analysis
- ISO 18184:2019 anti viral testing for textiles
- ISO 13629-2:2014
- ISO 21326:2019
- ASTM G21
Word Tags
AATCC 100, antibacterial textile testing, antimicrobial fabric testing, Staphylococcus aureus, Klebsiella pneumoniae, independent laboratory testing, ISO 17025
