ASHRAE 185.1-2020 UV Microbial Test Validation

Test Method for UV-C Disinfection in Air-Handling Units and Air Ducts

Scope

The ANSI/ASHRAE 185.1-2020 standard defines a test method for evaluating UV-C technologies in HVAC systems to determine their effectiveness in inactivating airborne microorganisms.

 The standard outlines key parameters, including:

  • Bioaerosol generation techniques
  • Upstream and downstream air sampling for microbial analysis
  • Environmental and airflow conditions necessary for standardized testing

 At Airmid Healthgroup, we have over a decade of experience testing a wide range of in-duct air purification technologies, including:

  • HEPA filters
  • Electrostatic filtration
  • UV-C disinfection systems
  • Photocatalytic oxidation (PCO)
  • Plasma-based air purification

 Our testing capabilities cover a broad spectrum of contaminants, including viruses, bacteria, mold, allergens, and volatile organic compounds (VOCs).

Our Testing Capabilities

We conduct standardized and customized evaluations using our modified ASHRAE type recirculation duct, designed to accommodate:

  • Single-pass testing for evaluating immediate filtration and disinfection performance
  • Multi-room recirculation testing for real-world performance simulation
  • Custom duct configurations tailored to specific product designs and applications

Test Overview

The ANSI/ASHRAE 185.1-2020 standard provides a rigorous test method to assess the effectiveness of UV-C lights in inactivating airborne microorganisms when installed in air-handling units (AHUs) or HVAC ductwork. The test simulates real-world conditions by introducing bioaerosols into a controlled test duct, where UV-C exposure is evaluated under defined airflow and environmental conditions.

Test Setup

  1. Test Duct Configuration:
  • A sealed, controlled test duct is used, typically based on an ASHRAE 52.2 test rig or a customized duct system that allows for precise control of airflow, temperature, and humidity.
  • The duct is equipped with a UV-C disinfection system, positioned according to the manufacturer’s specifications.

  1. Bioaerosol Generation:
  • Live airborne microorganisms (e.g., bacteria, viruses, or mold spores) are introduced into the duct using a nebulizer or bioaerosol generator.
  • The target bioaerosols are selected based on standard pathogenic models or customized based on the client’s product claims.

 

  1. Airflow & Environmental Conditions:
  • Airflow rates are set to mimic HVAC operational conditions, ensuring a realistic residence time for UV-C exposure.
  • Temperature and humidity levels are monitored and controlled, as these factors can influence microbial viability and UV-C efficacy.
Air Cleaner Performance Testing: Validating Efficacy Against Airborne Pathogens, Aerosols & Biomedical Research

Why Test with Airmid Healthgroup?

  • Expertise in Microbiology & Aerobiology – We specialize in evaluating airborne contaminants and their interaction with HVAC technologies.
  • Custom Test Design – In addition to ANSI/ASHRAE 185.1-2020 compliance, we develop bespoke testing protocols to meet the unique needs of your product.
  • Industry Credibility – Our results support product validation, regulatory submissions, and market claims for manufacturers worldwide.

Partner With Us

We can test your product in full compliance with ANSI/ASHRAE 185.1-2020, or collaborate with you to develop a customized evaluation that aligns with your specific goals.

Contact us today to discuss your testing requirements and ensure your air purification technology meets the highest industry standards.

Click Here

Scroll to Top