by Máire Fox MSc. et al, Airmid Health Group Ltd.,Trinity Enterprise Campus, Grand Canal Quay, Dublin 2, Ireland.
Abstract
Rationale: Allergen avoidance in the home should address contaminant reservoirs e.g.carpets and soft furnishings. The impact of a proprietary hot water extraction cleaning process on surface and airborne allergens and microorganisms was evaluated.
Methods: 20 US homes were studied. Air and surface samples taken before and after hot water extraction were analyzed for allergen and microorganism levels by ELISA and total viable counts. Airborne particle counts were monitored during room disturbances before and after cleaning. Carpet layers were analysed for particulate and allergen content by scanning electron microscopy and ELISA, respectively. Three carpets were similarly sampled in an environmental test chamber.
Results: Post‐hot water extraction cleaning, surface levels of Derp1/Derf1, Feld1 and Canf1 were reduced by 83%, 96% and 90% on soft furnishings (p = 0.04) and 91%,95% and 97% on carpets (p = 0.01), respectively.
Airborne Feld1 was reduced by 67%(p = 0.01). Reductions were also seen for airborne mold (55%, p = 0.01) and surface bacteria (90%, p = 0.05). Test chamber airborne particle counts during room disturbances in the presence of three different carpets were reduced post‐hot water extraction cleaning by 78%, 37% and 65%.
Allergen was predominantly found in the carpet base (≥71% ofFeld1/Canf1). Hot water extraction cleaning had a greater effect on allergen levels in the upper layers (74 ‐ 100% reduction), than on base layer levels (15 ‐ 91% reduction).
Conclusion: Incorporation of a hot water extraction cleaning procedure in a home allergen avoidance strategy, at yet to be determined intervals, significantly reduces the levels of bio‐contaminants to which occupants are exposed.
Field Study
The impact of a proprietary hot water extraction cleaning process on surface and airborne allergens and microorganisms was evaluated in 20 homes in a Midwestern state. Homes were selected on the basis ofhaving carpeting and domestic pets.
Test Chamber
Hot water extraction cleaning was performed on two out of four quadrants of three different carpets, i.e. two quadrants remained un‐cleaned.All quadrants were then removed from the homes and transported to a modified AC‐1/ASTM Test Chamber for testing as outlined above.
Field Study Results: Airborne Allergen
Airborne cat allergen levels were assessed during each testing stage. Pre‐ and post‐hot water extraction, a room disturbance (5 mins walking, then 5 mins bouncing a ball) was performed to disturb allergen into the air.
24 hours after hot water extraction, the levels of airborne cat allergen were 67% less than the levels detected prior to the cleaning process.

Field Study Result: Surface Allergen

Detectable levels of dust mite, cat and dog allergens were found in 4/20, 11/20 and15/20 homes respectively. 24 hours post‐hot water extraction, surface levels of allallergens were significantly less than those detected prior to the cleaning process (p ≤0.04)
Field Study Results: Airborne and Surface Microorganisms

Analysis of the concentration of bacteria present on the carpet surface demonstrated that 24 hours after hot water extraction, bacteria levels were 90% less than those detected prior to the cleaning process.

Test Chamber Results: Airborne Particles
Cleaned (hot water extracted) and un‐cleaned samples of three different used carpets were individually laid in the chamber. The air in the test chamber wasmonitored while disturbance activities were performed on the carpet samples.
Test Chamber Results: Carpet Layers
Lateral slices (top, middle & base layers) of the used carpet samples were analysed for their allergen levels. The carpet base contained >70% of the allergen content.
Conclusion
Hot water extraction cleaning had a significant impact on bio‐contaminants in the home, reducing the levels of allergen and microorganisms on carpet andsoft furnishings and in the air. Therefore the proprietary hot water extraction cleaning process investigated in this study was found to reduce the level of bio‐contaminant exposure to home occupants.
Based on the results of this study, hot water extraction cleaning would be a valuable addition to acomprehensive home allergen reduction plan. Regular vacuuming with a proven effective vacuum cleaner by the home owner, periodically supplemented at yet to be determined intervals by hot water extraction cleaning, can have a significant beneficial impact on allergen reservoirs in the home, even where carpets and soft furnishings remain.