JIS Z 2801

Description of the test

Antimicrobial Products – Test for antimicrobial activity and efficacy

Products that can be tested



  • Test organisms to be used are two stains of Escherichia coli (ATTC 25922/ATTC  8739) and two strains of Staphylococcus aureus (ATTC  6538/ ATTC 6538p) to be grown at 37˚C for 24 h and diluted to a working concentration of 5 x 105 cells/ml.
  • The control and test surfaces are inoculated with the working culture and covered with a sterile film ensuring close homogenous surface contact.
  • Bacterial viability at t = 0 is determined by serial dilution and plating out on an appropriate agar while untreated and treated samples are incubated at 37°C for 18 hours.
  • Samples are washed in TSB to extract bacteria from the surface and this extraction solution is serially diluted and plated out on an appropriate agar to visualise and enumerate colony forming units.
  • The elution method is validated by a control to ensure effective neutralization of the antimicrobial agent.
  • The efficacy of the antimicrobial compound is determined by calculating the log reduction in bacterial viability elicited by the test sample.

Third Party Validation

On passing this test, the tested product can be labelled as having been tested by airmid to JIS Z 2801 in support of any claim relating to the test and data received.

Turnaround Time

4 weeks

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